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4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)

  • Product Name: 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)
  • CAS: 7365-45-9
  • Purity:
  • Appearance: White crystalline powder

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What is the HEPES ?

HEPES is White crystalline powder, while it's Molecular Formula is C8H18N2O4S. HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research.HEPES has been used as a component of:Hank′s balanced salt solution, Dulbecco′s modified eagle′s medium and no-glucose DMEM, which are used for the preparation of tissue slices.Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity.

What is the CAS number for HEPES ?

The CAS number of HEPES is 7365-45-9.

More information of HEPES 7365-45-9 are:

Synonyms

1-[4-(2-Hydroxyethyl)-1-piperazinyl]ethane-2-sulfonicacid;2-[4-(2-Hydroxyethyl)piperazinyl]ethanesulfonic acid;4-(2-Hydroxyethyl)piperazine-1-(2-ethanesulfonic acid);N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid;N-(2-Hydroxyethyl)piperazine-N'-ethanesulfonic acid;NSC 166663;N'-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid);N'-2-Hydroxyethylpiperazine-N-2-ethanesulfonic acid;TVZ 7;WAS 13;HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid);1-Piperazineethanesulfonicacid, 4-(2-hydroxyethyl)-;

CAS Number

7365-45-9

Molecular Formula

C8H18N2O4S

Molecular Weight

238.308

Density

1.325 g/cm3

Melting Point

234-238 °C

Boiling Point

408℃[at 101 325 Pa]

HS CODE

DERIVATION

PSA

89.46000

LogP

-0.55930

Pka

7.5(at 25℃)

What is HEPES (7365-45-9) used for?

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

InChI:InChI=1/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)/p+1

Articles related to HEPES:

Article

Source

Preparation method of piperazine ethanesulfonic acid derivative (by machine translation)

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Paragraph 0048-0087, (2020/02/06)

High purity 4 - hydroxyethyl piperazine b sulfonic acid

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Paragraph 0051; 0052; 0060; 0061, (2017/09/26)

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